製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CK1 epsilon
  • 特異性
    ab70110 detects endogenous levels of total CK1 epsilon protein.
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human CK1 epsilon

  • ポジティブ・コントロール
    • LOVO cell extracts, HeLa cells and human placenta tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab70110 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • 組織特異性
    Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • 配列類似性
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Casein kinase 1 epsilon antibody
    • Casein kinase I antibody
    • Casein kinase I epsilon antibody
    • Casein kinase I isoform epsilon antibody
    • CKI epsilon antibody
    • CKI-epsilon antibody
    • CKIe antibody
    • CKIepsilon antibody
    • CSNK 1E antibody
    • CSNK1E antibody
    • DBT antibody
    • Doubletime, Drosophila, homolog of antibody
    • EC 2.7.11.1 antibody
    • epsilon isoform antibody
    • HCK IE antibody
    • KC1E antibody
    • KC1E_HUMAN antibody
    • kinase CK1 epsilon antibody
    • MGC 10398 antibody
    see all

画像

  • ab70110 at 1/500 dilution staining CK1 epsilon in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70110 at 1/50 dilution staining CK1 epsilon in human placenta by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
  • All lanes : Anti-CK1 epsilon antibody (ab70110) at 1/500 dilution

    Lane 1 : LOVO cell extracts
    Lane 2 : LOVO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa

参考文献

This product has been referenced in:
  • Lopez-Guerra JL  et al. High casein kinase 1 epsilon levels are correlated with better prognosis in subsets of patients with breast cancer. Oncotarget 6:30343-56 (2015). IHC . Read more (PubMed: 26327509) »
  • Scoma HD  et al. The De-Ubiquitinylating Enzyme, USP2, Is Associated with the Circadian Clockwork and Regulates Its Sensitivity to Light. PLoS One 6:e25382 (2011). WB ; Human . Read more (PubMed: 21966515) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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