IHC image of ab96600 staining in Human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96600, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Citrate synthetase antibody (ab96600)
All lanes : Anti-Citrate synthetase antibody (ab96600) at 1 µg/ml
Lane 1 : Marker Lane 2 : Zebrafish liver homogenate (20ug) Lane 3 : Zebrafish skeletal muscle homogenate (20ug) Lane 4 : Mouse liver mitochondrial homogenate (20ug)
Secondary Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution developed using the ECL technique