Citrate Synthase Activity Assay Kit (ab119692)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 15 min
- Sample type: Cell culture extracts, Tissue Extracts
- Sensitivity: 1.56 µg/ml
製品の概要
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製品名
Citrate Synthase Activity Assay Kit
Citrate synthetase キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 1 3 5.45% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 1 3 8.3% -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Enzyme activity -
検出感度
1.56 µg/ml -
検出範囲
1.56 µg/ml - 100 µg/ml -
全工程の試験時間
3h 15m -
種交差性
交差種: Cow, Human
非交差種: Mouse, Rat -
製品の概要
Citrate Synthase Activity Assay Kit (ab119692) is used to determine mitochondrial citrate synthase (CS) activity in a sample. The enzyme is immunocaptured within the wells of a microplate, and activity is determined by recording color development of TNB, which is generated in the reaction of citrate synthesis. The reaction product, TNB, absorbs at OD = 412 nm.
This kit immunocaptures only native citrate synthase from the samples. Using the immunocapture method allows the measurement of citrate synthase activity with simple sample preparation and no need for mitochondrial isolation.
This kit can be used with whole cell extracts or tissue homogenates.
Citrate synthase assay protocol summary:
- add samples to wells
- incubate for 3 hrs to capture enzyme
- wash with wash buffer
- add activity solution
- analyze with microplate reader for 5-30 min at 20s intervals -
特記事項
Citrate synthase (CS, O75390) is the initial enzyme of the tricarboxylic acid (TCA) cycle. This enzyme is a 51.7 kDa enzyme that catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate (EC 2.3.3.1). This enzyme is an exclusive marker of the mitochondrial matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Storage: All components are shipped cold. 10X Reaction Reagent mixture is shipped lyophilized. Before use, rehydrate the reaction mixture by adding 1mL base buffer. Vortex the tube thoroughly to dissolve the lyophilized powder to prepare 10X Reaction solution. After hydration unused amounts of the 10X Reaction solution should be stored at -80°C for 6 months. Store all other components at 4°C. This kit is stable for 6 months from receipt. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X Reaction Reagent 1 vial 1X Base Buffer 1 x 25ml 20X Buffer 1 x 20ml CS Microplate 1 unit Extraction Buffer (ab260490) 1 x 15ml -
研究分野
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パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
配列類似性
Belongs to the citrate synthase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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別名
- CISY_HUMAN
- Citrate synthase
- Citrate synthase, mitochondrial
see all -
参照データベース
- Entrez Gene: 280682 Cow
- Entrez Gene: 1431 Human
- Omim: 118950 Human
- SwissProt: Q29RK1 Cow
- SwissProt: O75390 Human
- Unigene: 430606 Human
- Unigene: 728168 Human
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab119692 は 28 報の論文で使用されています。
- Maunder E et al. Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males. Eur J Appl Physiol 122:93-102 (2022). PubMed: 34562114
- Mao L et al. Discovery of JND003 as a New Selective Estrogen-Related Receptor a Agonist Alleviating Nonalcoholic Fatty Liver Disease and Insulin Resistance. ACS Bio Med Chem Au 2:282-296 (2022). PubMed: 35874496
- Dong X et al. Silencing LINC00294 Restores Mitochondrial Function and Inhibits Apoptosis of Glioma Cells under Hypoxia via the miR-21-5p/CASKIN1/cAMP Axis. Oxid Med Cell Longev 2021:8240015 (2021). PubMed: 34777696
- Maunder E et al. Temperate performance and metabolic adaptations following endurance training performed under environmental heat stress. Physiol Rep 9:e14849 (2021). PubMed: 33977674
- Jourdain AA et al. Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from glycolysis to OXPHOS. Mol Cell 81:1905-1919.e12 (2021). PubMed: 33852893