製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Transcriptional coactivator of the p300/CBP-mediated trancription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner.
    • 組織特異性
      Expressed only in melanocytes and testis.
    • 配列類似性
      Belongs to the CITED family.
    • 翻訳後修飾
      Phosphorylated. Phosphorylation changes in a cell cycle-dependent manner and reduces its transcriptional coactivator activity.
    • 細胞内局在
      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm by a nuclear export signal and (NES) in a CRM1-dependent manner.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ABCC1 antibody
      • AI316840 antibody
      • AU019144 antibody
      • Cbp/p300-interacting transactivator 1 antibody
      • Cbp/p300-interacting transactivator with GLU/ASP-rich C-terminal domain, 1 antibody
      • Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1 antibody
      • CITE1_HUMAN antibody
      • CITED1 antibody
      • melanocyte specific gene 1 antibody
      • Melanocyte specific protein 1 antibody
      • Melanocyte-specific protein 1 antibody
      • MSG1 antibody
      see all

    画像



    • Predicted band size : 20 kDa
      CITED1 antibody (ab55467) at 1ug/lane + A-431 cell lysate at 25ug/lane.

    プロトコール

    参考文献

    ab55467 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Aug 01 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue (Kidney Tissue)
    Specification
    Kidney Tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Tracy Tran

    Verified customer

    投稿 Feb 10 2016

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (human kidney cells)
    Specification
    human kidney cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 18 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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