The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 10µg/mg of lysate.
WB: 1/2000 - 1/10000 (or 0.1 - 1µg/ml). Predicted molecular weight: 231 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CIT is a putative RHO/RAC effector that binds to the GTP bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. This protein exhibits dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system.
Rho interacting serine/threonine protein kinase 21 antibody
serine/threonine-protein kinase 21 antibody
Western blot - CIT antibody (ab86782)
All lanes : Anti-CIT antibody (ab86782) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 231 kDa
Exposure time : 30 seconds
Immunoprecipitation - CIT antibody (ab86782)
1 mg HeLa whole cell lysate was immunoprecipitated with 10 µg ab86782 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was loaded onto a Western blot and labelled with ab86782 at 1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.