製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CIRBP
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the C-terminal region of Human CIRBP (NP_001271.1).

  • ポジティブ・コントロール
    • Y79 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Contains 1 RRM (RNA recognition motif) domain.
  • ドメイン
    Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
  • 翻訳後修飾
    Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
    Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
  • 細胞内局在
    Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A18 hnRNP antibody
    • A18HNRNP antibody
    • cirbp antibody
    • CIRBP_HUMAN antibody
    • CIRP antibody
    • Cold inducible RNA binding protein antibody
    • Cold-inducible RNA-binding protein antibody
    • Glycine rich RNA binding protein antibody
    • Glycine-rich RNA-binding protein CIRP antibody
    • testicular tissue protein Li 39 antibody
    see all

Anti-CIRBP antibody 画像

  • ICC/IF image of ab94999 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94999 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CIRBP antibody (ab94999) at 1/100 dilution + Y79 cell line lysate at 35 µg

    Predicted band size : 19 kDa

Anti-CIRBP antibody (ab94999) 使用論文

This product has been referenced in:
  • Zhang Y  et al. Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner. Nucleic Acids Res 44:761-75 (2016). IF . Read more (PubMed: 26673712) »

See 1 Publication for this product

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The concentration of ab94999 CIRBP antibody lot number GR1103-8  is  0.5 mg/ml.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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