製品の概要

  • 製品名Anti-Cingulin antibody
    Cingulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cingulin
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 350-400 of Human Cingulin (NP_065821.1).

  • ポジティブ・コントロール
    • Jurkat, HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab117778 was affinity purified using an epitope specific to Cingulin immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab117778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
  • 組織特異性Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
  • 配列類似性Belongs to the cingulin family.
  • ドメインDeletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1.
  • 細胞内局在Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6330408J11Rik antibody
    • AI647528 antibody
    • AI987749 antibody
    • cgn antibody
    • CING_HUMAN antibody
    • Cingulin antibody
    • DKFZp779N1112 antibody
    • FLJ39281 antibody
    • KIAA1319 antibody
    • MGC118157 antibody
    see all

Anti-Cingulin antibody 画像

  • All lanes : Anti-Cingulin antibody (ab117778) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : Jurkat whole cell lysates at 50 µg
    Lane 4 : HeLa whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 136 kDa


    Exposure time : 3 minutes
  • Detection of Cingulin by Western Blot of Immunprecipitate.
    ab117778 at 1µg/ml staining Cingulin in 293T whole cell lysate immunoprecipitated using ab117778 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-Cingulin antibody (ab117778) 使用論文

ab117778 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"