製品の概要

  • 製品名Anti-CIITA antibody
    CIITA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CIITA
  • 特異性Detects human CIITA. Recognition is at the C-terminal region.
  • アプリケーション適用あり: Conjugation, Dot blot, ELISA, IP, WB, Flow Cytmore details
  • 免疫原

    Synthetic peptide:

    CGEIKDNKELPQYLALTTR

    conjugated to KLH - which corresponds to a region near the C-terminal end (amino acids 726-742) of the protein encoded by the human CIITA gene.CIITA.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by delipidation and defibrination.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Conjugation Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 123 kDa.
Flow Cyt Use at an assay dependent concentration. PubMed: 17586661

ターゲット情報

  • 機能Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
  • 関連疾患Bare lymphocyte syndrome 2
  • 配列類似性Contains 4 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • ドメインThe acetyltransferase domain is necessary for activation of both class I and class II transcription.
    The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
  • 翻訳後修飾Autophosphorylated, affecting interaction with TAF7.
  • 細胞内局在Nucleus. Nucleus, PML body. Recruited to PML body by PML.
  • Information by UniProt
  • 別名
    • C2TA antibody
    • C2TA_HUMAN antibody
    • CIITA antibody
    • CIITA IV antibody
    • Class II major histocompatibility complex transactivator antibody
    • Class II transactivator antibody
    • MHC class II transactivator antibody
    • MHC class II transactivator type III antibody
    • MHC2TA antibody
    • NLR family acid domain containing antibody
    • NLRA antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and acid domain containing antibody
    see all

Anti-CIITA antibody (ab7541) 使用論文

This product has been referenced in:
  • Christova R  et al. P-STAT1 mediates higher-order chromatin remodelling of the human MHC in response to IFNgamma. J Cell Sci 120:3262-70 (2007). WB ; Human . Read more (PubMed: 17726060) »
  • Herrmann TL  et al. MHC Class II levels and intracellular localization in human dendritic cells are regulated by calmodulin kinase II. J Leukoc Biol 82:686-99 (2007). Flow Cyt . Read more (PubMed: 17586661) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"