製品の概要

  • 製品名Anti-CIITA antibody
    CIITA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CIITA
  • 特異性This antibody recognizes CIITA.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Dog
  • 免疫原

    A region within synthetic peptide: MALWESLQQQ GEAQLLQAAE EKFTIEPFKA KSPKDVEDLD RLVQTQRLRN, corresponding to amino acids 918-967 of Mouse CIITA

  • ポジティブ・コントロール
    • NIH3T3 cell lysate
  • 特記事項


    Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 125 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
  • 関連疾患Bare lymphocyte syndrome 2
  • 配列類似性Contains 4 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • ドメインThe acetyltransferase domain is necessary for activation of both class I and class II transcription.
    The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
  • 翻訳後修飾Autophosphorylated, affecting interaction with TAF7.
  • 細胞内局在Nucleus. Nucleus, PML body. Recruited to PML body by PML.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C2TA antibody
    • C2TA_HUMAN antibody
    • CIITA antibody
    • CIITA IV antibody
    • Class II major histocompatibility complex transactivator antibody
    • Class II transactivator antibody
    • MHC class II transactivator antibody
    • MHC class II transactivator type III antibody
    • MHC2TA antibody
    • NLR family acid domain containing antibody
    • NLRA antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and acid domain containing antibody
    see all

Anti-CIITA antibody 画像

  • Anti-CIITA antibody (ab49132) at 0.125 µg/ml + NIH3T3 lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 125 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)

Anti-CIITA antibody (ab49132) 使用論文

ab49132 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab49132.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"