製品の概要

  • 製品名Anti-CIDE C antibody
    CIDE C 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CIDE C
  • アプリケーション適用あり: ELISA, Flow Cyt, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Pig
  • 免疫原

    Recombinant fragment: SVRKGIMAYS LEDLLLKVRD TLMLADKPFF LVLEEDGTTV ETEEYFQALA GDTVFMVLQK GQKWQPPSEQ GTRHPLSLSH KPAKKIDVA, corresponding to amino acids 53-141 of human CIDE C (NP_071377 or Q96AQ7) with a 26kDa tag.

  • ポジティブ・コントロール
    • HeLa cell lysate; recombinant tagged fragment of human CIDE C (the immunogen).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Induces apoptosis.
  • 組織特異性Expressed mainly in small intestine, heart, colon and stomach and, at lower levels, in brain, kidney and liver.
  • 配列類似性Contains 1 CIDE-N domain.
  • 細胞内局在Cytoplasm. Cytoplasmic in a punctate manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell death activator antibody
    • Cell death activator CIDE-3 antibody
    • Cell death inducing DFFA like effector C antibody
    • Cell death inducing DFFA like effector protein C antibody
    • Cell death-inducing DFFA-like effector protein C antibody
    • CIDE 3 antibody
    • CIDE3 antibody
    • CIDEC antibody
    • CIDEC_HUMAN antibody
    • Fat specific protein 27 antibody
    • Fat-specific protein FSP27 homolog antibody
    • FLJ20871 antibody
    • FPLD5 antibody
    • FSP27 antibody
    see all

Anti-CIDE C antibody 画像

  • Anti-CIDE C antibody (ab77115) at 1/500 dilution + recombinant tagged fragment of human CIDE C (the immunogen) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-CIDE C antibody (ab77115) at 1/500 dilution + Hela cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 27 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab77115 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HCT116 cells stained with ab77115 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77115, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min) /permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-CIDE C antibody (ab77115) 使用論文

This product has been referenced in:
  • Boren J & Brindle KM Apoptosis-induced mitochondrial dysfunction causes cytoplasmic lipid droplet formation. Cell Death Differ 19:1561-70 (2012). WB . Read more (PubMed: 22460322) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Cow Tissue lysate - whole (muscle, adipose tissue, liver)
Specification muscle, adipose tissue, liver
Blocking step Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Oct 22 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

Read More

Thank you for contacting us.

We currently have three anti-CLCN3 products on our catalog. Unfortunately these are not phospho specific antibody and will recognize CLCN3 regardless of phosphorylation state. I can suggest two courses of acti...

Read More

Thank you for taking time to provide this information and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"