Anti-cIAP2 抗体 [E40] (ab32059)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E40] to cIAP2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-cIAP2 antibody [E40]
cIAP2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E40] to cIAP2 -
由来種
Rabbit -
特異性
This antibody is specific for human cIAP2. It does not cross-react with any other IAP family members. -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human cIAP2 aa 400-500. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Daudi cell lysate IHC-P: Human spleen tissue, Human breast carcinomaIP: Daudi cell lysate Flow Cyt (intra): Raji cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E40 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32059の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/70.
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WB | (2) |
1/50000. Predicted molecular weight: 72 kDa.
For unpurified use at 1/1000. |
IHC-P |
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30.
For unpurified use at 5 µg/mL. |
特記事項 |
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Flow Cyt (Intra)
1/70. |
WB
1/50000. Predicted molecular weight: 72 kDa. For unpurified use at 1/1000. |
IHC-P
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. For unpurified use at 5 µg/mL. |
ターゲット情報
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機能
Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). -
組織特異性
Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes. -
関連疾患
Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma. -
配列類似性
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 330 Human
- Omim: 601721 Human
- SwissProt: Q13489 Human
- Unigene: 127799 Human
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別名
- AIP 1 antibody
- AIP1 antibody
- API 2 antibody
see all
画像
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Anti-cIAP2 antibody [E40] (ab32059) at 1/50000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa -
Anti-cIAP2 antibody [E40] (ab32059) at 1/1000 dilution (unpurified) + Daudi cell lysate.
Predicted band size: 72 kDa
Observed band size: 72 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling cIAP2 with purified ab32059 at 1/600 dilution (1.12 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling cIAP2 with purified ab32059 at 1/70 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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ab32059 (purified) at 1/30 dilution (2ug) immunoprecipitating cIAP2 in Daudi whole cell lysates.
Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates 10ug
Lane 2 (+): ab32059 & Daudi whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32059 in Daudi whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
ab32059 (unpurified) at a 1/50 dilution staining cIAP2 in human normal spleen, using Immunohistochemistry, Paraffin Embedded Tissue.
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cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5µg of Rabbit polyclonal to cIAP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32059 (unpurified).
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 72kDa; cIAP2
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (46)
ab32059 は 46 報の論文で使用されています。
- Wai CMM et al. Immune pathway upregulation and lower genomic instability distinguish EBV-positive nodal T/NK-cell lymphoma from ENKTL and PTCL-NOS. Haematologica 107:1864-1879 (2022). PubMed: 35021606
- Li N et al. Inhibitory effects of LOXL2 knockdown on cellular functions of liver cancer stem cells. Transl Cancer Res 11:2013-2025 (2022). PubMed: 35966289
- Sun H et al. cIAP1/2 are involved in the radiosensitizing effect of birinapant on NSCLC cell line in vitro. J Cell Mol Med N/A:N/A (2021). PubMed: 33939305
- Zhang X et al. Exploring the five different genes associated with PKCa in bladder cancer based on gene expression microarray. J Cell Mol Med 25:1759-1770 (2021). PubMed: 33452764
- Ren Y et al. Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis. Front Pharmacol 12:560903 (2021). PubMed: 33841136