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RabMAb

Anti-cIAP2 抗体 [E40] (ab32059)

製品の概要

  • 製品名
    Anti-cIAP2 antibody [E40]
    cIAP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E40] to cIAP2
  • 特異性
    This antibody is specific for human cIAP2. It does not cross-react with any other IAP family members.
  • アプリケーション
    適用あり: WB, IHC-P, ICC, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to the residues before the breakpoint for translocation to form BIRC3-MALT1 of Human cIAP2.

  • ポジティブ・コントロール
    • Daudi cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-cIAP2 antibody (HRP) [E40] (ab199501)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 72 kDa.
IHC-P Use at an assay dependent concentration.
ICC 1/100.
IP Use a concentration of 5 µg/ml.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
    • 組織特異性
      Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
    • 関連疾患
      Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
    • 配列類似性
      Belongs to the IAP family.
      Contains 3 BIR repeats.
      Contains 1 CARD domain.
      Contains 1 RING-type zinc finger.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AIP 1 antibody
      • AIP1 antibody
      • API 2 antibody
      • API2 antibody
      • Apoptosis inhibitor 2 antibody
      • Baculoviral IAP repeat containing 3 antibody
      • Baculoviral IAP repeat containing protein 3 antibody
      • Baculoviral IAP repeat-containing protein 3 antibody
      • BIRC 3 antibody
      • BIRC3 antibody
      • BIRC3_HUMAN antibody
      • C IAP2 antibody
      • C-IAP2 antibody
      • CIAP 2 antibody
      • CIAP2 antibody
      • HAIP 1 antibody
      • HAIP1 antibody
      • HIAP 1 antibody
      • HIAP-1 antibody
      • HIAP1 antibody
      • IAP homolog C antibody
      • IAP-1 antibody
      • Inhibitor of apoptosis protein 1 antibody
      • MALT 2 antibody
      • MALT2 antibody
      • Mammalian IAP homolog C antibody
      • MIHC antibody
      • RING finger protein 49 antibody
      • RNF49 antibody
      • TNFR2 TRAF signaling complex protein 1 antibody
      • TNFR2 TRAF signalling complex protein antibody
      • TNFR2-TRAF-signaling complex protein 1 antibody
      see all

    画像

    • Anti-cIAP2 antibody [E40] (ab32059) at 1/1000 dilution + Daudi cell lysate.

      Predicted band size : 72 kDa
      Observed band size : 72 kDa
    • ab32059 at a 1:50 dilution staining cIAP2 in human normal spleen, using Immunohistochemistry, Paraffin Embedded Tissue.
    • cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5µg of Rabbit polyclonal to cIAP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32059.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 72kDa; cIAP2

    参考文献

    This product has been referenced in:
    • Guan G  et al. Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells. Tumour Biol 39:1010428317691740 (2017). Read more (PubMed: 28347232) »
    • Guicciardi ME  et al. Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice. Cell Death Dis 8:e2535 (2017). IHC . Read more (PubMed: 28055006) »

    See all 21 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Epithelial)
    Specification
    Epithelial
    Treatment
    50 ng/TNF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 27 2014


    Please notice, these protocols may be optimised, and could not work with your specific tissue.

    Ab32059: Steam slides in 0.01M Sodium Citrate Buffer, pH 6.0 at 125°C for 30 seconds followed by 10 seconds at 90° C into a decloaking ...

    Read More
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10% acrylamyde)
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 04 2013

    Ok great, this will ship out tonight. Please keep me updated, and I look forward to hearing from you!

    Thanks for getting back to me. I'm sending a vial of ab23423 free of charge on the order ***, which we have in stock and can be delivered tomorrow. Will your receiving department be open tomorrow, or should I set it for a later delivery? Please keep me...

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    Thanks for your reply and for your patience while i have been in touch with the lab. I have heard back from the lab, and I don't see anything unusual in their protocol so I don't have a great explanation for these results. At this point, I would sugges...

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    Thank you for your reply and for sending the additional information. I am in touch with the testing laboratory to ask for further suggestions to get the correct results. I do have a few more questions, just to make sure I understand the images ...

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    Thanks for sending the image again. I'm sorry to see that the results are so poor with this replacement antibody. Do any of the bands on the blot correspond to the expected band size of cIAP2? How much lysate do you load into each well? The testing lab...

    Read More

    Thank you for keeping me updated about this situation and for sending the protocol information and image. I unfortunately can not open the Gel file. Could you re-send it in pdf or jpg? I will be happy to see if there are any suggestions that might impr...

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    Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending a free of charge replacement vial of ab113237 on the order ***, which I expect to arrive December 1. Please keep me update...

    Read More

    1-10 of 11 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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