製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to cIAP2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Available on request. (Peptide available as ab38273.)

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with cIAP2 peptide (ab38273).

ターゲット情報

  • 機能
    Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • 組織特異性
    Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
  • 関連疾患
    Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
  • 配列類似性
    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIP 1 antibody
    • AIP1 antibody
    • API 2 antibody
    • API2 antibody
    • Apoptosis inhibitor 2 antibody
    • Baculoviral IAP repeat containing 3 antibody
    • Baculoviral IAP repeat containing protein 3 antibody
    • Baculoviral IAP repeat-containing protein 3 antibody
    • BIRC 3 antibody
    • BIRC3 antibody
    • BIRC3_HUMAN antibody
    • C IAP2 antibody
    • C-IAP2 antibody
    • CIAP 2 antibody
    • CIAP2 antibody
    • HAIP 1 antibody
    • HAIP1 antibody
    • HIAP 1 antibody
    • HIAP-1 antibody
    • HIAP1 antibody
    • IAP homolog C antibody
    • IAP-1 antibody
    • Inhibitor of apoptosis protein 1 antibody
    • MALT 2 antibody
    • MALT2 antibody
    • Mammalian IAP homolog C antibody
    • MIHC antibody
    • RING finger protein 49 antibody
    • RNF49 antibody
    • TNFR2 TRAF signaling complex protein 1 antibody
    • TNFR2 TRAF signalling complex protein antibody
    • TNFR2-TRAF-signaling complex protein 1 antibody
    see all

プロトコール

参考文献

This product has been referenced in:
  • Lopergolo A  et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Br J Cancer 100:739-46 (2009). WB ; Mouse . Read more (PubMed: 19223905) »
  • Esposito I  et al. Overexpression of cellular inhibitor of apoptosis protein 2 is an early event in the progression of pancreatic cancer. J Clin Pathol 60:885-95 (2007). Read more (PubMed: 16775116) »

See all 4 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
25 µg
Specification
293T
Treatment
cIAP2 siRNA
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 17 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Nov 01 2005

Thank you for taking the time to submit your concerns and protocol, having now read through the information that you have sent me. There are a few suggestions that may be made. 1) From the gel the bands that you are observing in lanes two and three ...

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Thank you for your email. This antibody has been tested for cross-reactivity with both human and mouse, however we do not have any images available. If you have any more questions, please contact us again.

We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. This product falls into the latter category. The identity of our suppliers remains commercially sensitive i...

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The immunogen is 507-524 of human cIAP-2. The antibody has not been tested with rat sample, and has only been tested for application in Western blotting. Please refer to the online datasheet for ab2540 for any references that we have.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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