Anti-cIAP2 抗体 (ab137393)
Key features and details
- Rabbit polyclonal to cIAP2
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-cIAP2 antibody
cIAP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to cIAP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant fragment corresponding to Human cIAP2 aa 314-538.
Database link: Q13489 -
ポジティブ・コントロール
- WB: Rat spleen lysate. HeLa cells. U87-MG, SK-N-SH, IMR32, SK-N-AS whole cell extracts. IHC-P: Human SW480 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137393の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
ターゲット情報
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機能
Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). -
組織特異性
Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes. -
関連疾患
Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma. -
配列類似性
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 330 Human
- Entrez Gene: 78971 Rat
- Omim: 601721 Human
- SwissProt: Q13489 Human
- Unigene: 127799 Human
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別名
- AIP 1 antibody
- AIP1 antibody
- API 2 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue labelling cIAP2 with ab137393 at 1/100 dilution.
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All lanes : Anti-cIAP2 antibody (ab137393) at 1/1000 dilution
Lane 1 : U-87 MG whole cell extracts
Lane 2 : SK-N-SH whole cell extracts
Lane 3 : IMR32 whole cell extracts
Lane 4 : SK-N-AS whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa -
All lanes : Anti-cIAP2 antibody (ab137393) at 1/500 dilution
Lane 1 : HeLa whole cell extract
Lane 2 : HeLa cytosol extract
Lane 3 : HeLa nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE.
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Anti-cIAP2 antibody (ab137393) at 1/500 dilution + Rat spleen lysate at 50 µg
Predicted band size: 68 kDa7.5% SDS-PAGE.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab137393 は 4 報の論文で使用されています。
- Pilling AB et al. IAP Antagonists Enhance Apoptotic Response to Enzalutamide in Castration-Resistant Prostate Cancer Cells via Autocrine TNF-α Signaling. Prostate 77:866-877 (2017). PubMed: 28240376
- Zhang S et al. Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma. Oncotarget 5:7183-97 (2014). PubMed: 25216531
- Wagner L et al. Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-?B-dependent manner. Oncogene : (2012). PubMed: 22469979
- Bangert A et al. Histone deacetylase inhibitors sensitize glioblastoma cells to TRAIL-induced apoptosis by c-myc-mediated downregulation of cFLIP. Oncogene 31:4677-88 (2012). PubMed: 22266862