Anti-Chromogranin C 抗体 [SGII 6B1/3] (ab20245)




Our Abpromise guarantee covers the use of ab20245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
ICC/IF Use at an assay dependent concentration.
ICC Use a concentration of 2 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 機能Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides.
  • 配列類似性Belongs to the chromogranin/secretogranin protein family.
  • 細胞内局在Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CHGC antibody
    • Chromogranin-C antibody
    • EM66 antibody
    • SCG2 antibody
    • SCG2_HUMAN antibody
    • Secretogranin II antibody
    • Secretogranin-2 antibody
    • Secretoneurin antibody
    • SGII antibody
    • SN antibody
    see all

Anti-Chromogranin C antibody [SGII 6B1/3] 画像

  • Confocal image of immunofluorescence staining with Mouse monoclonal [SGII 6B1/3] to Chromogranin C;(ab20245; 2µg/ml for 1h at room temperature) in red and nuclear counterstain in blue on Rat Cell (Hippocampal neuron culture). Cells were fixed with paraformaldehyde; BSA (5%) was used as a blocking agent (40 minutes at room temperature). Secondary antibody was Goat anti-mouse IgG2b Alexa Fluor 568 Conjugation (1/600).

  • IHC image of ab20245 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20245, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Chromogranin C antibody [SGII 6B1/3] (ab20245) 使用論文

This product has been referenced in:
  • Oh JY  et al. Identification of the HSPB4/TLR2/NF-?B axis in macrophage as a therapeutic target for sterile inflammation of the cornea. EMBO Mol Med : (2012). ICC/IF ; Rat . Read more (PubMed: 22359280) »
  • Kim CH  et al. Norepinephrine deficiency is caused by combined abnormal mRNA processing and defective protein trafficking of dopamine beta-hydroxylase. J Biol Chem 286:9196-204 (2011). WB . Read more (PubMed: 21209083) »

See all 4 Publications for this product

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Of the Chromogranin C antibodies we have, ab20245 will be the best non-rabbit-host choice. It has not been tested for reactivity with mouse samples but the immunogen sequence, derived from rat protein, is 100% co...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Hippocampal neuron culture)
Specification Hippocampal neuron culture
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%

Dr. Randal Moldrich

Verified customer

投稿 May 17 2006