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Synthetic peptide conjugated to Ovalbumin, corresponding to N terminal amino acids 1-19 of Chromogranin C
Our Abpromise guarantee covers the use of ab12241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 17933551|
|WB||1/250 - 1/500. Predicted molecular weight: 74 kDa.|
|IP||1/25 - 1/100.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
ab12241 staining Chromogranin C in Mouse primary cortical neuronal cells treated with IL-1β by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked with 10% serum for 1 hours at room temperature°C. Samples were incubated with primary antibody (1/1000 in 0.3% BSA) for 1 hour at room temperature. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.