製品の概要

  • 製品名Anti-Chromogranin C antibody
    Chromogranin C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chromogranin C
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-FoFr, IHC-Fr, ICCmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to Ovalbumin, corresponding to N terminal amino acids 1-19 of Chromogranin C

  • 特記事項Dilute in PBS or medium which is identical to that used in the assay system.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 17933551
WB 1/250 - 1/500. Predicted molecular weight: 74 kDa.
IP 1/25 - 1/100.
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr 1/100.
ICC Use at an assay dependent concentration.

ターゲット情報

Anti-Chromogranin C antibody 画像

  • ab12241 staining Chromogranin C in Mouse primary cortical neuronal cells treated with IL-1β by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked with 10% serum for 1 hours at room temperature°C. Samples were incubated with primary antibody (1/1000 in 0.3% BSA) for 1 hour at room temperature. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.

Anti-Chromogranin C antibody (ab12241) 使用論文

This product has been referenced in:
  • Blanco EH  et al. An amphipathic alpha-helix in the prodomain of cocaine and amphetamine regulated transcript peptide precursor serves as its sorting signal to the regulated secretory pathway. PLoS One 8:e59695 (2013). ICC/IF ; Rat . Read more (PubMed: 23527253) »
  • Kuczewski N  et al. Backpropagating action potentials trigger dendritic release of BDNF during spontaneous network activity. J Neurosci 28:7013-23 (2008). ICC/IF ; Rat . Read more (PubMed: 18596175) »

See all 4 Publications for this product

Product Wall

This antibody has been tested on frozen sections of human pituitary. We do not know if it will work on formalin-fixed, paraffin-embedded tissue sections. Ab12241 has also been tested on monolayer cultures of a pituitary tumor cell line, GH4C1, for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"