製品の概要

  • 製品名Anti-Chromogranin B antibody
    Chromogranin B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chromogranin B
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human Chromogranin B.

  • ポジティブ・コントロール
    • WB: Human adrenal gland tissue lysate and PC12 (untreated and NGF treated) whole cell lysates. IHC-P: Rat normal brain and human normal pancreas tissues. ICC/IF: PC12 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
  • 組織特異性Expressed in the adrenal medulla, and in pheochromocytoma. Not expressed in liver.
  • 配列類似性Belongs to the chromogranin/secretogranin protein family.
  • 翻訳後修飾Extensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues.
  • 細胞内局在Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCB peptide antibody
    • Cg B antibody
    • CgB antibody
    • Chgb antibody
    • Chromogranin-B antibody
    • ChromograninB antibody
    • SCG1 antibody
    • SCG1_HUMAN antibody
    • Secretogranin 1 antibody
    • Secretogranin B antibody
    • Secretogranin I antibody
    • SgI antibody
    see all

Anti-Chromogranin B antibody 画像

  • All lanes : Anti-Chromogranin B antibody (ab85286) at 1 µg/ml

    Lane 1 : Human adrenal normal tissue lysate - total protein (ab29249)
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) NGF-Treated Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • IHC image of Chromogranin B staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85286, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab85286 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85286, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Chromogranin B antibody (ab85286) 使用論文

ab85286 has not yet been referenced specifically in any publications.

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