Anti-Chromogranin A 抗体 [LK2H10], prediluted (ab8204)


  • 製品名Anti-Chromogranin A antibody [LK2H10], prediluted
    Chromogranin A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LK2H10] to Chromogranin A, prediluted
  • 特異性The antibody is specific for chromogranin A. Specificity was ascertained by immunoblotting and immunohistochemistry. The antibody predominantly reacts with a 68 kDa protein.
  • アプリケーション適用あり: WB, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human, Pig
  • 免疫原

    Antigen was isolated from human Pheochromocytoma.

  • エピトープHuman. Crossreactivity with monkey and pig.
  • ポジティブ・コントロール
    • Adrenal medulla or pancreas tissue.



Our Abpromise guarantee covers the use of ab8204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. PubMed: 19041781
IHC-FoFr 1/10.
IHC-Fr 1/10. Acetone fixed.
IHC-P 1/10.


  • 機能Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • 配列類似性Belongs to the chromogranin/secretogranin protein family.
  • 翻訳後修飾Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • 細胞内局在Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • 参照データベース
  • 製品の状態According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • 別名
    • beta Granin antibody
    • CgA antibody
    • CHG A antibody
    • Chga antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    see all

Anti-Chromogranin A antibody [LK2H10], prediluted 画像

  • ab8204 staining Chromogen A in normal Human colon mucosa tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation with citrate buffer. Samples were incubated with primary antibody (1/10 in TBS) for 1 hour. An undiluted HRP-conjugated Goat polyclonal was used as the secondary antibody.

    See Abreview

Anti-Chromogranin A antibody [LK2H10], prediluted (ab8204) 使用論文

This product has been referenced in:
  • Moran AW  et al. Sweet taste receptor expression in ruminant intestine and its activation by artificial sweeteners to regulate glucose absorption. J Dairy Sci 97:4955-72 (2014). IHC-Fr ; Sheep, Cow . Read more (PubMed: 24881785) »
  • Moran AW  et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). IHC-Fr ; Pig . Read more (PubMed: 20338074) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon normal mucosa)
Specification colon normal mucosa
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Karin Birkenkamp-Demtroeder

Verified customer

投稿 Aug 13 2010

Thank you for your enquiry. The concentration of this antibody is 18 ug/ml. In addition, I noticed a mistake on the datasheet. The antibody is not purified. I have updated the datasheet to reflect this and have included a link for your convenience...

Read More

Thank you for your enquiry. For ab8204, the concentration is 15-16 ug/ml. For ab8205, we are sorry to inform you that there is no protein concentration for polyclonal antibodies that are not affinity purified.This antibody is provided as whole serum an...

Read More

The concentration is 15-16 microgram/ml. The product has a four day lead time so should be in stock four days from when the order is placed.