Anti-Chromogranin A 抗体 [LK2H10 + PHE5] (ab715)

製品の概要

  • 製品名
    Anti-Chromogranin A antibody [LK2H10 + PHE5]
    Chromogranin A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LK2H10 + PHE5] to Chromogranin A
  • アプリケーション
    適用あり: ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
  • 免疫原

    Human pheochromocytoma

  • エピトープ
    Although the epitopes for these two clones are not precisely mapped, experimental data suggest that they are different.
  • ポジティブ・コントロール
    • Pancreas, adrenal gland, bowel and thyroid
  • 特記事項
    This antibody is a mixture of two clones, LK2H10 and PHE5.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • 配列類似性
    Belongs to the chromogranin/secretogranin protein family.
  • 翻訳後修飾
    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • 細胞内局在
    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • 別名
    • beta Granin antibody
    • betagranin (N-terminal fragment of chromogranin A) antibody
    • catestatin antibody
    • CgA antibody
    • CHG A antibody
    • Chga antibody
    • chromofungin antibody
    • Chromogranin A antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • Secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    see all

Anti-Chromogranin A antibody [LK2H10 + PHE5] 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of neuroendocrine tumor tissue labeling Chromogranin A with ab715.

  • Immunocytochemistry/ Immunofluorescence analysis of human prostate cells labeling Chromogranin A with ab715 at 1/200 dilution (green). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.8% Triton X-100. A donkey anti-mouse (Alexa Fluor® 647) was used as the secondary antibody at 1/400 dilution.

    See Abreview

Anti-Chromogranin A antibody [LK2H10 + PHE5] (ab715) 使用論文

This product has been referenced in:
  • Burke AP  et al. Carcinoids of the jejunum and ileum: an immunohistochemical and clinicopathologic study of 167 cases. Cancer 79:1086-93 (1997). Read more (PubMed: 9070484) »
  • Angelsen A  et al. Neuroendocrine differentiation in carcinomas of the prostate: do neuroendocrine serum markers reflect immunohistochemical findings? Prostate 30:1-6 (1997). Read more (PubMed: 9018329) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.8% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Jan 04 2008

Thank you for your enquiry and patience. I have confirmed with the laboratory that the ab715 antibody's concentration is 200ug/ml and the purification is by protein G chromatography. I hope this information will be useful to you. If you require a...

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Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused. If you have any questions in th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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