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This antibody is a mixture of two clones, LK2H10 and PHE5.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab45179 as a replacement.
Our Abpromise guarantee covers the use of ab715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of neuroendocrine tumor tissue labeling Chromogranin A with ab715.
Immunocytochemistry/ Immunofluorescence analysis of human prostate cells labeling Chromogranin A with ab715 at 1/200 dilution (green). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.8% Triton X-100. A donkey anti-mouse (Alexa Fluor® 647) was used as the secondary antibody at 1/400 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"