Anti-Chromogranin A 抗体 [EP1031Y] (ab52983)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1031Y] to Chromogranin A
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Chromogranin A antibody [EP1031Y]
Chromogranin A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1031Y] to Chromogranin A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide within Human Chromogranin A (C terminal). The exact sequence is proprietary.
(Peptide available asab138920) -
ポジティブ・コントロール
- PC12 cytoplasmic cell lysate Human pancreatic tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解離定数(KD 値)
KD = 6.90 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1031Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52983の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1e+006. Detects a band of approximately 80-90 kDa (predicted molecular weight: 51 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1e+006. Detects a band of approximately 80-90 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
配列類似性
Belongs to the chromogranin/secretogranin protein family. -
翻訳後修飾
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
細胞内局在
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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参照データベース
- Entrez Gene: 1113 Human
- Entrez Gene: 24258 Rat
- Omim: 118910 Human
- SwissProt: P10645 Human
- SwissProt: P10354 Rat
- Unigene: 150793 Human
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製品の状態
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
別名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
画像
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ab52983, at a 1/250 dilution, staining Human Chromogranin A in Pancreas using Immunohistochemistry, Paraffin Emebedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-Chromogranin A antibody [EP1031Y] (ab52983) at 1/1000000 dilution + PC12 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 80-90 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab52983 は 4 報の論文で使用されています。
- Rao VKS et al. Multiple roles for peptidylglycine a-amidating monooxygenase in the response to hypoxia. J Cell Physiol N/A:N/A (2021). PubMed: 34061983
- Hu J et al. Pitfalls in the management of subglottic paragangliomas at unusual location: a case report and literature review. BMC Surg 21:340 (2021). PubMed: 34496808
- Thomsen SK et al. Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic ß-cells. Nat Genet 50:1122-1131 (2018). PubMed: 30054598
- Simpson PD et al. Striking Oxygen Sensitivity of the Peptidylglycine a-Amidating Monooxygenase (PAM) in Neuroendocrine Cells. J Biol Chem 290:24891-901 (2015). WB ; Human . PubMed: 26296884