製品の概要
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製品名Anti-Chromogranin A antibody
Chromogranin A 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Chromogranin A
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WB, IP, ICC/IFmore details
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種交差性交差種: Mouse, Human, Monkey
交差が予測される動物種: Rat, Horse, Cow, Dog, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Chromogranin A.
(Peptide available as ab93753.)
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ポジティブ･コントロール
- This antibody gave a positive signal in human pancreas tissue lysate. This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell line:PANC-1.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab85554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). | |
| IP | Use a concentration of 5 µg/ml. | |
| ICC/IF | Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
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配列類似性Belongs to the chromogranin/secretogranin protein family.
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翻訳後修飾Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
細胞内局在Secreted. Neuroendocrine and endocrine secretory granules.
- Information by UniProt
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参照データベース
- Entrez Gene: 281070 Cow
- Entrez Gene: 100033828 Horse
- Entrez Gene: 1113 Human
- Entrez Gene: 12652 Mouse
- Entrez Gene: 397540 Pig
- Entrez Gene: 24258 Rat
- Omim: 118910 Human
- SwissProt: P05059 Cow
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製品の状態According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
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別名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
画像
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Immunoprecipitation - Anti-Chromogranin A antibody (ab85554)Chromogranin A was immunoprecipitated using 0.5mg Mouse Pancreas tissue lysate, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Pancreas tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85554.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 50kDa; Chromogranin A
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Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody (ab85554)ICC/IF image of ab85554 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85554 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Chromogranin A antibody (ab85554)Anti-Chromogranin A antibody (ab85554) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Chromogranin A antibody (ab85554)IHC image of Chromogranin A staining in Human Pancreas Adenocarcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85554, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab85554)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC image of Chromogranin A staining in Monkey Pancreas sections, performed The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6). Sections were subjected to 2% H202O then Avidin-Biotin to block endogenous peroxidases/biotin. The section was then incubated with ab85554, 1/5000, for 2 hours at 12°C. The section was then counterstained with haematoxylin
プロトコール
参考文献
This product has been referenced in:
- Terry NA et al. Impaired enteroendocrine development in intestinal-specific Islet1 mouse mutants causes impaired glucose homeostasis. Am J Physiol Gastrointest Liver Physiol 307:G979-91 (2014). Read more (PubMed: 25214396) »
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