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Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
|1000X Protease Inhibitor Cocktail||1 x 110µl|
|10X Lysis Buffer||1 x 11ml|
|Chromatin Buffer||1 x 11ml|
|Extraction Buffer||1 x 11ml|
Our Abpromise guarantee covers the use of ab117152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Chromatin yield was 4 µg chromatin/106 cells.Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments