製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CHRFAM7A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide derived from residues 11 - 60 of human CHRFAM7A.

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab28885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 - 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. CHRFAM7A is encoded by a hybrid gene consisting of a duplication of exons 5 through 10 of the CHRNA7 gene fused 3-prime to a copy of the FAM7A gene (exons A through E). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel.
  • 細胞内局在
    Membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • Alpha 7 neuronal nicotinic acetylcholine receptor FAM7A hybrid antibody
    • CHRNA7 (cholinergic receptor nicotinic alpha 7 exons 5 10) and FAM7A (family with sequence similarity 7A exons A E) fusion antibody
    • CHRNA7 antibody
    • CHRNA7 DR1 antibody
    • CHRNA7 FAM7A fusion antibody
    • CHRNA7 FAM7A fusion protein antibody
    • D 10 antibody
    • D10 antibody
    • MGC120482 antibody
    • MGC120483 antibody
    see all

画像

  • Anti-CHRFAM7A antibody (ab28885) at 0.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG (suggested dilution 1:50000 - 100000)
    Developed using the ECL technique

    Predicted band size : 45 - 56 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab28885 has not yet been referenced specifically in any publications.

レビューと Q&A

This is correct, the Uniprot reference for the human CHRFAM7A is Q494W8. Please note that the exact immunogen is proprietary, the information given on the datasheet (aa 11-60) is the region where the exact sequence is derived from. According to the...

Read More

Upon Blasting the peptide immunogen used to generate this antibody we found that it is 100% homologous to the human CHRNA7 protein (NCBI gene ID: 1139) and 100% homologous to the fusion protein CHRFAM7A (NCBI gene ID: 89832).  The next highest homolo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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