Anti-CHREBP 抗体 (HRP) (ab81958)

製品の概要

  • 製品名Anti-CHREBP antibody (HRP)
    CHREBP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CHREBP (HRP)
  • 標識HRP
  • 特異性A customer has observed some cross-reactivity with MLX-interacting protein (Q9HAP2). Although the exact sequence is proprietary, the immunogen for ab81958 shows 75% homology with this protein.
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human CHREBP aa 800-852 (C terminal). The exact sequence is proprietary.
    Database link: NP_116569.1

  • ポジティブ・コントロール
    • Human hepatocyte lysate, human hepatocytes, liver nuclear extracts from well fed rats, and colon epithelium.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 95 kDa.
IHC-P Use at an assay dependent dilution.
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'.
  • 組織特異性Expressed in liver, heart, kidney, cerebellum and intestinal tissues.
  • 関連疾患Note=WBSCR14 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR14 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • carbohydrate response element binding protein antibody
    • bHLHd14 antibody
    • Carbohydrate responsive element binding protein antibody
    • Class D basic helix-loop-helix protein 14 antibody
    • MIO antibody
    • MLX interacting protein like antibody
    • Mlx interactor antibody
    • MLX-interacting protein-like antibody
    • MLXIPL antibody
    • MONDOB antibody
    • WBS14_HUMAN antibody
    • WBSCR 14 antibody
    • WBSCR14 antibody
    • Williams Beuren syndrome chromosome region 14 antibody
    • Williams Beuren syndrome chromosome region 14 protein antibody
    • Williams-Beuren syndrome chromosomal region 14 protein antibody
    • WS basic helix loop helix leucine zipper protein antibody
    • WS basic-helix-loop-helix leucine zipper protein antibody
    • WS bHLH antibody
    • WS-bHLH antibody
    see all

Anti-CHREBP antibody (HRP) 画像

  • anti CHREBP (unconjugated form of ab81958), staining CHREBP in colon epithelium (40X) by immunohistochemistry.
  • anti CHREBP (unconjugated form of ab81958) + human hepatocyte lysate at 20 µg

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • anti CHREBP (unconjugated form of ab81958), at a 1/100 dilution, staining CHREBP in human hepatocyte by Immunofluorescence. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).
  • All lanes : anti CHREBP (unconjugated form of ab81958), at 1/1000 dilution

    Lane 1 : liver nuclear extracts from well fed rats at 10 µg
    Lane 2 : liver nuclear extracts from well fed rats at 5 µg
    Lane 3 : liver nuclear extracts from well fed rats at 1 µg
    Lane 4 : liver nuclear extracts from well fed rats at 0.5 µg


    Predicted band size : 95 kDa
    Observed band size : 95 kDa

Anti-CHREBP antibody (HRP) (ab81958) 使用論文

ab81958 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver and fat tissue)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 50 µg
Specification liver and fat tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Feb 17 2016


Pour visualiser l’HRP il faut ajouter un substrat qui, lorsqu'il est oxydé par l'HRP, change de couleur ou produit de la lumière qui peut être détecté.
De nombreux substrats pour HRP peuvent être utilisés :
HRP catalyse la conversion des...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"