製品の概要

  • 製品名Anti-CHRDL1 antibody
    CHRDL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CHRDL1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-450 of Human CHRDL1 (ENSP00000361115)

  • ポジティブ・コントロール
    • Mouse kidney tissue lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embyonic bone formation (By similarity). May also play an important role in regulating retinal angiogenesis trough modulation of BMP4 actions in endothelial cells.
  • 組織特異性Expressed in retinal pericytes.
  • 配列類似性Contains 3 VWFC domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CHL antibody
    • chordin like 1 antibody
    • Chordin-like protein 1 antibody
    • CHRDL1 antibody
    • CRDL1_HUMAN antibody
    • dA141H5.1 antibody
    • neuralin 1 antibody
    • Neuralin-1 antibody
    • Neurogenesin-1 antibody
    • NRLN1 antibody
    • Ventroptin antibody
    • VOPT antibody
    see all

Anti-CHRDL1 antibody 画像

  • ICC/IF image of ab103369 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103369 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CHRDL1 antibody (ab103369) at 1/500 dilution + Mouse kidney tissue lysate

    Predicted band size : 51 kDa
  • All lanes : Anti-CHRDL1 antibody (ab103369) at 1/500 dilution

    Lane 1 : CHRDL1 transfected 293T cell lysate
    Lane 2 : 293T non-transfected cell lysate


    Predicted band size : 51 kDa

Anti-CHRDL1 antibody (ab103369) 使用論文

ab103369 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab103369.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"