製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Chordin
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Horse, Cow, Dog, Macaque monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Chordin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human, Mouse and Rat brain; Mouse and Rat liver.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 102 kDa).

ターゲット情報

画像

  • All lanes : Anti-Chordin antibody (ab101316) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate
    Lane 5 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 46 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

プロトコール

参考文献

ab101316 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Chicken Cell (Whole Embryo)
Specification
Whole Embryo
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投稿 May 08 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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