Anti-Chondroitin Sulfate 抗体 [CS-56] (ab11570)

製品の概要

  • 製品名
    Anti-Chondroitin Sulfate antibody [CS-56]
  • 製品の詳細
    Mouse monoclonal [CS-56] to Chondroitin Sulfate
  • 特異性
    The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
  • アプリケーション
    適用あり: IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Chicken, Cow, Human, Apteronotus leptorhynchus
  • 免疫原

    Ventral membranes of chicken gizzard fibroblasts.

  • ポジティブ・コントロール
    • Bovine mammary gland epithelial (BMGE) cells.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR288674, GR272407. Lot numbers higher than GR288674, GR272407 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    Many cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200. PubMed: 16237170
The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution.

画像

  • ab11570 staining Chondroitin Sulfate in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    For more detail please see abreview.

    See Abreview

  • ab11570 staining Chondroitin Sulfate in 16 µm thick transverse sections of Apteronotus leptorhynchus whole body tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in 2% paraformaldehyde, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab11570 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-mouse polyclonal used at a 1/200 dilution.

    See Abreview

  • Immunoperoxidase staining of a frozen section of normal rabbit aorta stained using Monoclonal Anti-Chondroitin Sulfate ab11570.
  • Normal rabbit aorta, Lowicryl K4M thin section, stained with Monoclonal Anti-Chondroitin Sulfate, ab11570 and Goat Anti-Mouse IgM (µ-chain specific) 10 nm gold. Counterstain was uranyl acetate and Reynold's lead citrate. Magnification 44,600x. (el=Elastin, col=Collagen).

参考文献

This product has been referenced in:
  • Sidhaye J & Norden C Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis. Elife 6:N/A (2017). Zebrafish . Read more (PubMed: 28372636) »
  • Li X  et al. Transplantation of hUC-MSCs seeded collagen scaffolds reduces scar formation and promotes functional recovery in canines with chronic spinal cord injury. Sci Rep 7:43559 (2017). Read more (PubMed: 28262732) »

See all 13 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

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Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Nov 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Whole body)
Specification
Whole body
Fixative
Paraformaldehyde
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
microwave euthanasia
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trilogy
Permeabilization
Yes - tween20 (0.05%)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Adrian Epstein

Verified customer

投稿 Aug 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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