The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR288674, GR272407. Lot numbers higher than GR288674, GR272407 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Many cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. PubMed: 16237170 The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
Use at an assay dependent concentration.
Use at an assay dependent dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)Image courtesy of Adrian Epstein by Abreview.
ab11570 staining Chondroitin Sulfate in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). For more detail please see abreview.
Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)Image courtesy of Dr Ruxandra Sirbulescu by Abreview.
ab11570 staining Chondroitin Sulfate in 16 µm thick transverse sections of Apteronotus leptorhynchus whole body tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in 2% paraformaldehyde, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab11570 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-mouse polyclonal used at a 1/200 dilution.
Sidhaye J & Norden C Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis. Elife6:N/A (2017).
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Li X et al. Transplantation of hUC-MSCs seeded collagen scaffolds reduces scar formation and promotes functional recovery in canines with chronic spinal cord injury. Sci Rep7:43559 (2017).
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