製品の概要

  • 製品名
    Choline/Acetylcholine Assay Kit
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
  • アッセイタイプ
    Quantitative
  • 検出感度
    > 0.01 nmol/well
  • 検出範囲
    0.01 nmol/well - 5 nmol/well
  • 全工程の試験時間
    0h 40m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    Choline/Acetylcholine Assay Kit (ab65345) provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline – free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect ~10 pmol-5 nmol of choline or acetylcholine.


    Cells should be cultured in choline chloride free medium.

    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine (AchE), a critical neurotransmitter in both the periferial and central nervous systems.

    If you need to purchase additional Choline Probe for this product, please see ab139505.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

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  • Acetylcholine levels from mouse brain tissue was measured using Acetylcholine assay kit (ab65345). Normal mice were anesthetized with thiopental sodium at 20minutes after the end of  electroacupuncture (EA) treatment to determine levels of ACh (Figure A). Immunohistochemical staining  mAChR (muscarinic acetylcholine receptor) M3 in mouse cerebral cortex tissue 20 minutes after the end of EA treatment (Image B).

  • Choline measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • Choline measured fluorometrically in cell lysates showing quantity (pmol) per 1 mln of tested cells

  • Choline measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample

プロトコール

参考文献

This product has been referenced in:
  • Reale M  et al. Butyrylcholinesterase and Acetylcholinesterase polymorphisms in Multiple Sclerosis patients: implication in peripheral inflammation. Sci Rep 8:1319 (2018). Human . Read more (PubMed: 29358722) »
  • Pickard JM  et al. Intrinsic cardiac ganglia and acetylcholine are important in the mechanism of ischaemic preconditioning. Basic Res Cardiol 112:11 (2017). Read more (PubMed: 28091727) »

See all 8 Publications for this product

レビューと Q&A

J’ai contacté le laboratoire concernant les limites de détection de ce kit et de sa sensibilité.
Comme montré sur la courbe standard pour la détection colorimétrique, la limite de détection inf...

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I suggest running a time course to see how much time is needed for your samples to show a linear increase and then plateau off. It is recommended to choose the final incubation time within this linear increase regime.
Also, please note it is impor...

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ab65345 cannot be used for in-cell assay. It is meant for cell lysates.

Thank you for your email and your interest in our products.

I can confirm that you are right. It will be necessary to set up 2 different sets of wells for measuring the total choline and the free choline in this assay.

Therefore, to m...

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Thank you for contacting us.

We have not tested this kit with rat samples. However this kit should work as it does not contain any human specific antibody. The species is irrelevant for this product.

It will also be a good idea to inc...

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Thank you for contacting us.

All of the information and images we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Unfortunatel...

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Thank you very much for contacting Abcam. Your details has been forwarded to me by my colleague Peter.

The kit ab65345 have a detection range of 0.01 nmol/well to 5 nmol/ml; expression outside of this range will be undetectablewith the kit. <...

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Thank you for pointing this out. The datasheet is somewhat mis-leading with regards to the 500-fold range that is specified, and also in error with regards to the notation. I apologize for the confusion.

The specified range uses the low valu...

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Tissue culture SN can be used with this assay. Add up to 50 µl of the SN to wells and make up the final volume in all wells to 50 µl with the assay buffer. Continue with the protocol.

Thank you for your email. I am sorry to hear that you have been experiencing problems with this kit.

I have checked the details you have kindly provided; however I would like to ask few questions in order to understand the problem;

...

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1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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