Anti-Choline kinase alpha 抗体 - Aminoterminal end (ab38290)

製品の概要

  • 製品名
    Anti-Choline kinase alpha antibody - Aminoterminal end
    Choline kinase alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Choline kinase alpha - Aminoterminal end
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 50~150 of human Choline kinase alpha.

  • ポジティブ・コントロール
    • Mouse heart tissue lysate.
  • 特記事項


    This antibody recognises the N-terminal region of CHK.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    This antibody recognises the N-terminal region of CHK.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
ELISA 1/1000.

ターゲット情報

  • 関連性
    The dominant pathway for biosynthesis of phosphatidylcholine occurs through the CDP-choline pathway. Choline kinase alpha (CHK) is the first enzyme in the pathway and may thereby play an upstream regulatory role in lipid transport and metabolism. CHK additionally catalyzes phosphorylation of ethanolamine.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • CHETK alpha antibody
    • CHETK-alpha antibody
    • CHK antibody
    • CHKA antibody
    • CK antibody
    • CKI antibody
    • EK antibody
    • Ethanolamine kinase antibody
    see all

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プロトコール

参考文献

This product has been referenced in:
  • Arlauckas SP  et al. Near infrared fluorescent imaging of choline kinase alpha expression and inhibition in breast tumors. Oncotarget 8:16518-16530 (2017). Read more (PubMed: 28157707) »
  • Huang C  et al. Activation of choline kinase by extracellular Ca2+ is Ca(2+)-sensing receptor, Galpha12 and Rho-dependent in breast cancer cells. Cell Signal 21:1894-900 (2009). WB ; Human . Read more (PubMed: 19716891) »

See all 3 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell line)
Loading amount
30 µg
Specification
breast cancer cell line
Gel Running Conditions
Reduced Denaturing (8-16%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dorit Grunberger

Verified customer

投稿 Aug 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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