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RabMAb

Anti-Choline Acetyltransferase 抗体 [EPR16590] (ab178850)

製品の概要

  • 製品名
    Anti-Choline Acetyltransferase antibody [EPR16590]
    Choline Acetyltransferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR16590] to Choline Acetyltransferase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Guinea pig
  • 免疫原

    Synthetic peptide within Pig Choline Acetyltransferase aa 150-250. The exact sequence is proprietary.
    Database link: P13222
    (Peptide available as ab203108)

  • ポジティブ・コントロール
    • WB: Rat brain lysate; Rabbit brain lysate; Guinea Pig brain and cerebellum lysates. IHC-P: Mouse cerebral cortex tissue, Guinea Pig cerebral cortex tissue,
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR16590
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab178850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能
    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • 関連疾患
    Myasthenic syndrome, congenital, 6, presynaptic
  • 配列類似性
    Belongs to the carnitine/choline acetyltransferase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

画像

  • Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • All lanes : Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/5000 dilution

    Lane 1 : Guinea Pig brain lysate
    Lane 2 : Guinea Pig cerebellum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal. 

    See Abreview

  • Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution + Rabbit brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

参考文献

This product has been referenced in:

See all 2 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 Tris-EDTA
Permeabilization
No
Specification
Brain
Blocking step
Protein Block (ab64226) as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
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投稿 Jan 09 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin
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投稿 Oct 21 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spinal Cord)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% PBS-X
Specification
Spinal Cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Periodate/lysine/paraformaldehyde
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投稿 Feb 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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