Anti-Choline Acetyltransferase 抗体 - C-terminal (ab85609)

製品の概要

  • 製品名Anti-Choline Acetyltransferase antibody - C-terminal
    Choline Acetyltransferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Choline Acetyltransferase - C-terminal
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Rat Choline Acetyltransferase.

    (Peptide available as ab97420.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Rat Cortex and Mouse Cholinergic nucleus Tissue Lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

ターゲット情報

  • 機能Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • 関連疾患Myasthenic syndrome, congenital, 6, presynaptic
  • 配列類似性Belongs to the carnitine/choline acetyltransferase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Anti-Choline Acetyltransferase antibody - C-terminal 画像

  • Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cortex (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cholinergic nucleus Mouse Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 76 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) 使用論文

This product has been referenced in:
  • Moghimi E  et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One 10:e0126355 (2015). WB ; Mouse . Read more (PubMed: 26020962) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 04 2013

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"