Anti-Choline Acetyltransferase 抗体 - C-terminal (ab85609)
Key features and details
- Rabbit polyclonal to Choline Acetyltransferase - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Choline Acetyltransferase antibody - C-terminal
Choline Acetyltransferase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Choline Acetyltransferase - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Rat Choline Acetyltransferase aa 600 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab97420) -
ポジティブ・コントロール
- This antibody gave a positive signal in both Rat Cortex and Mouse Cholinergic nucleus Tissue Lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85609の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). |
ターゲット情報
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機能
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. -
関連疾患
Myasthenic syndrome, congenital, 6, presynaptic -
配列類似性
Belongs to the carnitine/choline acetyltransferase family. - Information by UniProt
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参照データベース
- Entrez Gene: 12647 Mouse
- Entrez Gene: 290567 Rat
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 442817 Mouse
- Unigene: 45116 Rat
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別名
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- ChAT antibody
see all
画像
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Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cortex (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cholinergic nucleus Mouse Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab85609 は 2 報の論文で使用されています。
- Xu J et al. Self-Assembling Peptide Scaffold Carrying Neural-Cell Adhesion Molecule-Derived Mimetic-Peptide Transplantation Promotes Proliferation and Stimulates Neurite Extension by Modulating Tau Phosphorylation and Calpain/Glycogen Synthase Kinase 3 beta (GSK-3ß) in Neurons. Ann Transplant 25:e924093 (2020). PubMed: 32686658
- Moghimi E et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One 10:e0126355 (2015). WB ; Mouse . PubMed: 26020962