This antibody appears to have identical characteristics to those described by Benecke et al (1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both peripheral and central nervous system, staining both cell bodies and nerve terminals.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. See Abreview.
Use at an assay dependent concentration. PubMed: 19422789
1/2000. Fix using formaldehyde. Perfusion fix the tissue with Zamboni fixative and post-fix (1-2hrs according to Masliukov PM & Timmermans) in 4% formaldehyde (4% formaldehyde with or without 15% picric acid is a very good fixative, it should also work with Bouin fixative too).Cryoprotect the tissue by direct immersion in 30% sucrose (at +4C). Use on free floating sections for best results. This antibody does not work with glutaraldehyde fixation.
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Myasthenic syndrome, congenital, 6, presynaptic
Belongs to the carnitine/choline acetyltransferase family.
Immunohistochemistry analysis of free-floating rat forebrain tissue labelling Choline Acetyltransferase with ab6168 at a dilution of 1/100.
Immunohistochemistry (Frozen sections) - Anti-Choline Acetyltransferase antibody (ab6168)This image is courtesy of an abreview submitted by Marcin Arciszewski
ab6168 staining rat stomach tissue sections by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde. The primary antibody was diluted 1/200 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal (IgG whole molecule) to rabbit IgG, diluted 1/400 was used as secondary.