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Cholesterol/ Cholesteryl Ester Quantitation Kit (ab65359) provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both by colorimetric or fluorometric methods. Majority of the cholesterol in blood is in the form of cholesteryl esters, which can be hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield H2O2 which reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).
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|Cholesterol Assay Buffer||WM||1 x 25ml|
|Cholesterol Esterase (lyophilized)||Blue||1 vial|
|Cholesterol Probe (in DMSO, anhydrous)||Red||1 x 200µl|
|Cholesterol Standard (2 µg/µl )||Yellow||1 x 100µl|
|Enzyme Mix (lyophilized)||Green||1 vial|
Our Abpromise guarantee covers the use of ab65359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Cholesterol and cholesteryl ester were quantified colourimetrically in biological fluids (dilution range 1:10-1:100; 50 minutes incubation) and measured in duplicates (+/- SD).