製品の概要

  • 製品名Anti-Cholera Toxin antibody [B041M]
    Cholera Toxin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B041M] to Cholera Toxin
  • 特異性ab62970 reacts with cholera toxin beta subunit.
  • アプリケーション適用あり: ELISAmore details
  • 免疫原

    Purified beta subunit of Cholera Toxin.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)>90% pure (SDS-PAGE).
  • ポリ/モノモノクローナル
  • クローン名B041M
  • ミエローマSp2/0
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 関連性The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
  • 細胞内局在Secreted
  • 別名
    • ctxA antibody
    • Cholera enterotoxin B chain antibody
    • Cholera enterotoxin gamma chain antibody
    • Cholera enterotoxin subunit A antibody
    • Cholera enterotoxin subunit B antibody
    • Choleragenoid antibody
    • ctxA antibody
    • ctxB antibody
    • toxA antibody
    • toxB antibody
    see all

Anti-Cholera Toxin antibody [B041M] (ab62970) 使用論文

ab62970 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"