製品の概要

  • 製品名Anti-CHMP6 antibody
    CHMP6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CHMP6
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CHMP6.

    (Peptide available as ab90851.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Hela; Jurkat; HepG2; HEK293; MCF7; JEG3; K562.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 23 kDa).

ターゲット情報

  • 機能Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the SNF7 family.
  • ドメインThe acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • 細胞内局在Endomembrane system. Endosome membrane. Late endosome membrane. Localizes to endosomal membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Charged multivesicular body protein 6 antibody
    • chmp6 antibody
    • CHMP6_HUMAN antibody
    • Chromatin modifying protein 6 antibody
    • Chromatin-modifying protein 6 antibody
    • FLJ11749 antibody
    • hVps 20 antibody
    • hVps20 antibody
    • novel SNF7 protein antibody
    • OTTMUSP00000004363 antibody
    • RGD1565325 antibody
    • RP24 225K18.4 antibody
    • Vacuolar protein sorting associated protein 20 antibody
    • Vacuolar protein sorting-associated protein 20 antibody
    • Vps 20 antibody
    • Vps20 antibody
    see all

Anti-CHMP6 antibody 画像

  • All lanes : Anti-CHMP6 antibody (ab76929) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

Anti-CHMP6 antibody (ab76929) 使用論文

ab76929 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

投稿 Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"