Anti-CHMP5 抗体 (ab96273)
Key features and details
- Rabbit polyclonal to CHMP5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CHMP5 antibody -
製品の詳細
Rabbit polyclonal to CHMP5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Zebrafish -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 172 of Human CHMP5
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ポジティブ・コントロール
- 293T, A431, H1299, HeLa S3, HepG2 whole cell lysate (ab7900) (WB). NCIN87 xenograft (IHC).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
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IHC-P |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. -
配列類似性
Belongs to the SNF7 family. -
細胞内局在
Cytoplasm > cytosol. Endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body. - Information by UniProt
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参照データベース
- Entrez Gene: 51510 Human
- Entrez Gene: 76959 Mouse
- Entrez Gene: 393351 Zebrafish
- Omim: 610900 Human
- SwissProt: Q9NZZ3 Human
- SwissProt: Q9D7S9 Mouse
- SwissProt: Q7T339 Zebrafish
- Unigene: 635313 Human
see all -
別名
- apoptosis-related protein PNAS-2 antibody
- C9orf83 antibody
- CGI 34 antibody
see all
画像
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All lanes : Anti-CHMP5 antibody (ab96273) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ab96273 at 1/100 dilution
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96273 は 1 報の論文で使用されています。
- Son F et al. Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting. Methods Mol Biol 1998:219-226 (2019). PubMed: 31250305