製品の概要

  • 製品名
    Anti-CHMP2A antibody
  • 製品の詳細
    Mouse polyclonal to CHMP2A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-222 of Human CHMP2A

  • ポジティブ・コントロール
    • Transfected 293T cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab67064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
    • 配列類似性
      Belongs to the SNF7 family.
    • ドメイン
      The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
    • 細胞内局在
      Late endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BC 2 antibody
      • BC2 antibody
      • Charged multivesicular body protein 2a antibody
      • CHM2A_HUMAN antibody
      • CHMP2 antibody
      • CHMP2a antibody
      • Chromatin modifying protein 2a antibody
      • Chromatin-modifying protein 2a antibody
      • hVps2 1 antibody
      • hVps2-1 antibody
      • putative breast adenocarcinoma marker antibody
      • Putative breast adenocarcinoma marker BC-2 antibody
      • Putative breast adenocarcinoma marker BC2 antibody
      • Vacuolar protein sorting associated protein 2 1 antibody
      • Vacuolar protein sorting-associated protein 2-1 antibody
      • Vps2 1 antibody
      • VPS2 antibody
      • Vps2-1 antibody
      • VPS2A antibody
      see all

    画像

    • All lanes : Anti-CHMP2A antibody (ab67064) at 1/500 dilution

      Lane 1 : CHMP2A transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 25 kDa
      Observed band size : ~28 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab67064 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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