製品の概要

  • 製品名Anti-CHMP2A antibody
    CHMP2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CHMP2A
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 169-218 (DELGLSLTDE LSNLPSTGGS LSVAAGGKKA EAAASALADA DADLEERLKN) of Human CHMP2A (NP_055268).

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーConstituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
  • 配列類似性Belongs to the SNF7 family.
  • ドメインThe acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • 細胞内局在Late endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BC2 antibody
    • Charged multivesicular body protein 2a antibody
    • CHM2A_HUMAN antibody
    • CHMP2 antibody
    • CHMP2a antibody
    • Chromatin modifying protein 2a antibody
    • Chromatin-modifying protein 2a antibody
    • hVps2 1 antibody
    • hVps2-1 antibody
    • putative breast adenocarcinoma marker antibody
    • Putative breast adenocarcinoma marker BC-2 antibody
    • Putative breast adenocarcinoma marker BC2 antibody
    • Vacuolar protein sorting associated protein 2 1 antibody
    • Vacuolar protein sorting-associated protein 2-1 antibody
    • Vps2 1 antibody
    • VPS2 antibody
    • Vps2-1 antibody
    • VPS2A antibody
    see all

Anti-CHMP2A antibody 画像

  • Anti-CHMP2A antibody (ab118729) at 1 µg/ml + 293T cell lysate at 10 µg

    Predicted band size : 25 kDa

Anti-CHMP2A antibody (ab118729) 使用論文

ab118729 has not yet been referenced specifically in any publications.

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Thank you for supplying us with this extra information about the protocol you used. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

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Looking at the western blot, there is a strong and prominent band after 1 min of exposure using ab118729. This band is at 25kDa on the blot you sent me, is this not the molecular weight that it should be? From my research, I've found that a band a...

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