Anti-Chloride Channel 5 抗体 (ab97482)

製品の概要

  • 製品名Anti-Chloride Channel 5 antibody
    Chloride Channel 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chloride Channel 5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Guinea pig, Pig
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 62-363 of Human chloride channel 5 (NP_000075)

  • ポジティブ・コントロール
    • A431 whole cell lysate, HepG2 and Raji lysates.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.

ターゲット情報

  • 機能Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function.
  • 組織特異性Kidney. Moderately expressed in aortic vascular smooth muscle and endothelial cells, and at a slightly higher level in the coronary vascular smooth muscle.
  • 関連疾患Hypophosphatemic rickets, X-linked recessive
    Nephrolithiasis 2
    Nephrolithiasis 1
    Low molecular weight proteinuria with hypercalciuria and nephrocalcinosis
  • 配列類似性Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily.
    Contains 2 CBS domains.
  • 翻訳後修飾Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.
  • 細胞内局在Golgi apparatus membrane. Endosome membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Chloride channel protein 5 antibody
    • Chloride channel voltage sensitive 5 antibody
    • Chloride transporter ClC-5 antibody
    • ClC-5 antibody
    • CLC5 antibody
    • CLCK2 antibody
    • CLCN5 antibody
    • CLCN5_HUMAN antibody
    • DENTS antibody
    • H(+)/Cl(-) exchange transporter 5 antibody
    • hCIC-K2 antibody
    • NPHL1 antibody
    • NPHL2 antibody
    • Voltage gated chloride ion channel CLCN5 antibody
    • XLRH antibody
    • XRN antibody
    see all

Anti-Chloride Channel 5 antibody 画像

  • Anti-Chloride Channel 5 antibody (ab97482) at 1/500 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 83 kDa

Anti-Chloride Channel 5 antibody (ab97482) 使用論文

ab97482 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (LLCPK1)
Permeabilization Yes - 0.3% Triton in PBS
Specification LLCPK1
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Sufyan Ali Sayyed

Verified customer

投稿 Oct 05 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (LLCPK1)
Gel Running Conditions Reduced Denaturing (7.5)
Loading amount 50 µg
Specification LLCPK1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Sufyan Ali Sayyed

Verified customer

投稿 Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"