製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CHL1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Mouse CHL1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following mouse tissue lysates: Cerebellum; Spinal Cord; Retina; Optic Nerve.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 135 kDa).

ターゲット情報

  • 関連性
    CHL1 is an extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. CHL1 plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. CHL1 may play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins and recruits ANK3 to the plasma membrane.
  • 細胞内局在
    Cell Membrane and Secreted
  • 参照データベース
  • 別名
    • CALL antibody
    • Cell adhesion molecule with homology to L1CAM (close homolog of L1) antibody
    • Cell adhesion molecule with homology to L1CAM (close homologue of L1) antibody
    • Cell adhesion molecule with homology to L1CAM antibody
    • Close homolog of L1 antibody
    • FLJ44930 antibody
    • L1 cell adhesion molecule 2 antibody
    • L1CAM2 antibody
    • MGC132578 antibody
    • Neural cell adhesion molecule antibody
    see all

画像

  • All lanes : Anti-CHL1 antibody (ab93197) at 1 µg/ml

    Lane 1 : Cerebellum (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Retina (Mouse) Tissue Lysate
    Lane 4 : Optic Nerve (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 135 kDa
    Observed band size : 220 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa,140 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

ab93197 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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