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corresponding to Human Chk2.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab32148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/20 - 1/50.|
|WB||1/1000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 61 kDa).|
Blocking/dilution buffer: 5% NFDM/TBST
ab32148 at 1/20 dilution immunoprecipitating Chk2 (phospho T68) in HEK-293 (human embryonic kidney) whole cell lysate observed at 70kDa (lanes 1 and 2).
Lane 1 (input): HEK-293 cells treated with UV at 60J/m2 for 2 hours. Whole cell lysate 10μg
Lane 2 (+): ab32148 + HEK-293 treated with UV at 60J/m2 for 2 hours. Whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32148 in HEK- treated with UV at 60J/m2 for 2 hours. Whole cell lysate
For western blotting, ab32148 at 1/500 dilution was used followed by ab131366 VeriBlot for IP (HRP) at 1/1000 as the secondary antibody.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Non-specific band : 35, 30 kDa
Blocking/Diluting buffer : 5% Milk
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"