製品の概要

  • 製品名
    Anti-Chk2 (phospho T68) antibody
    Chk2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chk2 (phospho T68)
  • 特異性
    ab3501 was tested using MCF7 cells treated with different amounts of doxorubicin (a DNA damaging agent). As the treatment increased the WB signal at 60kDa representing Chk2 increased, indicating that ab3501 was detecting the phosphorylated form of the protein. It has also been shown by peptide ELISA to be specific for the phosphorylated form of the immunizing peptide versus the non-phosphorylated form.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chimpanzee
  • 免疫原

    Synthetic peptide: (C)ETVSTpQELYS, corresponding to amino acids 64-73 of Chk2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

ターゲット情報

  • 機能
    Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • 組織特異性
    High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • 関連疾患
    Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • 配列類似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylated by PLK4.
  • 細胞内局在
    Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDS 1 antibody
    • Cds1 antibody
    • Cds1 homolog antibody
    • Checkpoint kinase 2 antibody
    • Checkpoint like protein CHK2 antibody
    • CHEK 2 antibody
    • Chek2 antibody
    • Chk 2 antibody
    • CHK2 checkpoint homolog (S. pombe) antibody
    • CHK2 checkpoint homolog antibody
    • CHK2_HUMAN antibody
    • hCds1 antibody
    • HuCds 1 antibody
    • LFS 2 antibody
    • LFS2 antibody
    • PP1425 antibody
    • RAD 53 antibody
    • RAD53 antibody
    • Rad53 homolog antibody
    • Serine/threonine protein kinase Chk2 antibody
    • Serine/threonine-protein kinase Chk2 antibody
    see all

Anti-Chk2 (phospho T68) antibody 画像



  • Predicted band size : 61 kDa

    The Western blot shows lysates from MCF-7 cells treated for 24 hours with increasing concentrations of the DNA damaging agent Doxorubicin (0-10µM). The blot clearly shows that Chk2 phosphorylation is increased with the higher concentrations of Doxorubicin.

プロトコール

Anti-Chk2 (phospho T68) antibody (ab3501) 使用論文

This product has been referenced in:
  • Kim SH  et al. Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity. Oncotarget 8:5026-5037 (2017). WB ; Human . Read more (PubMed: 27926513) »
  • Sidler C  et al. A role for SUV39H1-mediated H3K9 trimethylation in the control of genome stability and senescence in WI38 human diploid lung fibroblasts. Aging (Albany NY) 6:545-63 (2014). WB . Read more (PubMed: 25063769) »

See all 4 Publications for this product

Product Wall

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (Neural stem cells)
Specification
Neural stem cells
Treatment
MMS 0,02% Adriamicine 0,5 ´g /mL
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. J Igual

Verified customer

投稿 May 08 2014

Thank you very much for your interest in our antibodies against Chk2 phospho Thr68.
To our knowledge, all these three antibodies have not been tested in rodent species. All these three antibodies are however predicted to react against the mouse an...

Read More

Thank you for your enquiry. We have not received any feedback from customers who have tried this antibody for cross-reactivity with mouse. Although it is predicted to cross-react due to 90% sequence homology, we cannot guarantee it. Should you decid...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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