Anti-Chk2 (phospho T68) 抗体 (ab3501)
Key features and details
- Rabbit polyclonal to Chk2 (phospho T68)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Chk2 (phospho T68) antibody
Chk2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Chk2 (phospho T68) -
由来種
Rabbit -
特異性
ab3501 was tested using MCF7 cells treated with different amounts of doxorubicin (a DNA damaging agent). As the treatment increased the WB signal at 60kDa representing Chk2 increased, indicating that ab3501 was detecting the phosphorylated form of the protein. It has also been shown by peptide ELISA to be specific for the phosphorylated form of the immunizing peptide versus the non-phosphorylated form. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chimpanzee -
免疫原
Synthetic peptide corresponding to Chk2 aa 50-150 (phospho T68).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3501の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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特記事項 |
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WB
1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
ターゲット情報
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機能
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. -
組織特異性
High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. -
関連疾患
Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500]. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
Contains 1 FHA domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated by PLK4. -
細胞内局在
Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53. - Information by UniProt
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参照データベース
- Entrez Gene: 11200 Human
- Entrez Gene: 50883 Mouse
- Entrez Gene: 114212 Rat
- Omim: 604373 Human
- SwissProt: O96017 Human
- SwissProt: Q9Z265 Mouse
- Unigene: 291363 Human
- Unigene: 505297 Human
see all -
別名
- CDS 1 antibody
- Cds1 antibody
- Cds1 homolog antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab3501 は 10 報の論文で使用されています。
- Lan B et al. CRISPR-Cas9 Screen Identifies DYRK1A as a Target for Radiotherapy Sensitization in Pancreatic Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35053488
- Dunlop CR et al. Complete loss of ATM function augments replication catastrophe induced by ATR inhibition and gemcitabine in pancreatic cancer models. Br J Cancer 123:1424-1436 (2020). PubMed: 32741974
- Wang J et al. Bacterial quorum-sensing signal IQS induces host cell apoptosis by targeting POT1-p53 signalling pathway. Cell Microbiol 21:e13076 (2019). PubMed: 31254473
- Pan Z et al. Cinobufagin Induces Cell Cycle Arrest at the G2/M Phase and Promotes Apoptosis in Malignant Melanoma Cells. Front Oncol 9:853 (2019). PubMed: 31552178
- Wang D et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep 25:398-412.e6 (2018). PubMed: 30304680
- Kim SH et al. Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity. Oncotarget 8:5026-5037 (2017). WB ; Human . PubMed: 27926513
- Higashiguchi M et al. Clarifying the biological significance of the CHK2 K373E somatic mutation discovered in The Cancer Genome Atlas database. FEBS Lett 590:4275-4286 (2016). PubMed: 27716909
- Sidler C et al. A role for SUV39H1-mediated H3K9 trimethylation in the control of genome stability and senescence in WI38 human diploid lung fibroblasts. Aging (Albany NY) 6:545-63 (2014). WB . PubMed: 25063769
- Bertout JA et al. HIF2{alpha} inhibition promotes p53 pathway activity, tumor cell death, and radiation responses. Proc Natl Acad Sci U S A 106:14391-6 (2009). WB ; Human . PubMed: 19706526
- Chabalier-Taste C et al. BRCA1 is regulated by Chk2 in response to spindle damage. Biochim Biophys Acta 1783:2223-33 (2008). PubMed: 18804494