製品の概要

  • 製品名Anti-Chk2 antibody [DCS-270]
    Chk2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DCS-270] to Chk2
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Recombinant Human Chk2.

  • ポジティブ・コントロール
    • HeLa cell lysates; Human intestine tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab115149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.2 µg/ml. Predicted molecular weight: 61 kDa.
IP Use a concentration of 6.6 - 10 µg/ml.
IHC-P Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • 組織特異性High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • 関連疾患Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated by PLK4.
  • 細胞内局在Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDS 1 antibody
    • Cds1 antibody
    • Cds1 homolog antibody
    • Checkpoint kinase 2 antibody
    • Checkpoint like protein CHK2 antibody
    • CHEK 2 antibody
    • Chek2 antibody
    • Chk 2 antibody
    • CHK2 checkpoint homolog (S. pombe) antibody
    • CHK2 checkpoint homolog antibody
    • CHK2_HUMAN antibody
    • hCds1 antibody
    • HuCds 1 antibody
    • LFS 2 antibody
    • LFS2 antibody
    • PP1425 antibody
    • RAD 53 antibody
    • RAD53 antibody
    • Rad53 homolog antibody
    • Serine/threonine protein kinase Chk2 antibody
    • Serine/threonine-protein kinase Chk2 antibody
    see all

Anti-Chk2 antibody [DCS-270] 画像

  • All lanes : Anti-Chk2 antibody [DCS-270] (ab115149) at 0.2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : NIH3T3 cell lysate
    Lane 3 : Rat1 cell lysate

    Developed using the ECL technique

    Predicted band size : 61 kDa
  • Detection of Chk2 by Western Blot of Immunprecipitate.
    ab115149 at 0.2µg/ml staining Chk2 in HeLa cell lysate immunoprecipitated using anti-Chk2 at 10ug/ml (lane 2). Lane 1: IgG control.
  • ab115149 at 5ug/ml staining Chk2 in Paraffin-embedded Human intestine tissue by Immunohistochemistry.

Anti-Chk2 antibody [DCS-270] (ab115149) 使用論文

ab115149 has not yet been referenced specifically in any publications.

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