Anti-Chk1 (phospho S345) 抗体 (ab58567)

製品の概要

  • 製品名Anti-Chk1 (phospho S345) antibody
    Chk1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chk1 (phospho S345)
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Chk1.

    (Peptide available as ab93794.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Hela whole cell lysate. IHC-P: FFPE Human cervix normal.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

Anti-Chk1 (phospho S345) antibody 画像

  • All lanes : Anti-Chk1 (phospho S345) antibody (ab58567) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Chk1 (phospho S345) peptide (ab93794) at 1 µg/ml
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Chk1 peptide (ab116685) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • IHC image of ab58567 staining Human Cervical Carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58567, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ICC/IF image of ab58567 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58567, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.

Anti-Chk1 (phospho S345) antibody (ab58567) 使用論文

ab58567 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cell line)
Loading amount 40 µg
Specification HeLa cell line
Treatment mitomycin C
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Sep 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount 15 µg
Specification MRC5VA Lung Epithelial Fibroblasts
Treatment 30GY Ionising Radiation
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Apr 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"