製品の概要

  • 製品名Anti-Chk1 (phospho S345) antibody
    Chk1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chk1 (phospho S345)
  • アプリケーション適用あり: ICC/IF, IHC-Fr, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Drosophila melanogaster
  • 免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue
  • 特記事項


    Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 一次抗体 備考Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab47318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200. See Abreview.
IHC-Fr Use at an assay dependent concentration. PubMed: 20978077Fixed in -20°C methanol.
IHC-P 1/50 - 1/100.
ELISA 1/10000.

ターゲット情報

Anti-Chk1 (phospho S345) antibody 画像

  • ab47318 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
  • ab47318 (1/200) detecting Chk1 in HeLa cells in conjunction with a goat anti-rabbit secondary antibody conjugated to Cy3® (red). Cells were also counterstained with DAPI in order to highlight the nucleus (blue), treated with Bleomycin and incubated with an antibody against Histone H2AX in order to create and expose DNA double strand breaks (green). Please refer to abreviews for further details.

    See Abreview

Anti-Chk1 (phospho S345) antibody (ab47318) 使用論文

This product has been referenced in:
  • Wang CY  et al. DNA-PK/Chk2 induces centrosome amplification during prolonged replication stress. Oncogene N/A:N/A (2014). Read more (PubMed: 24662822) »
  • Zhou J  et al. A kinome screen identifies checkpoint kinase 1 (CHK1) as a sensitizer for RRM1-dependent gemcitabine efficacy. PLoS One 8:e58091 (2013). Human . Read more (PubMed: 23483975) »

See all 8 Publications for this product

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Thank you for completing our recent survey. As you have noted technical difficulties with two antibodies from Abcam, I just wanted to follow up to see how I might help to rectify any issues.
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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount 15 µg
Specification MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Darren Arbon

Verified customer

投稿 Apr 14 2011

Thank you for your enquiry. All the information that we have about the protocol used by K. McManus is already stated on the review. At Abcam, we always give our customers all the information that we have at hand and I am afraid that there is no othe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Apr 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"