Anti-Chk1 (phospho S280) 抗体 (ab59988)

製品の概要

  • 製品名
    Anti-Chk1 (phospho S280) antibody
    Chk1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chk1 (phospho S280)
  • アプリケーション
    適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acid residues surrounding S280 region of human Chk1, conjugated to KLH

  • ポジティブ・コントロール
    • A2058, Ramos, Jurkat, HL60, Hela cells or mouse brain lysates. Human hepatocarcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    After protein G affinity chromatography, the antibody fraction was peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • Information by UniProt
  • 参照データベース
  • 別名
    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

Anti-Chk1 (phospho S280) antibody 画像

  • All lanes : Anti-Chk1 (phospho S280) antibody (ab59988) at 1/100 dilution

    Lane 1 : A2058 cell lysate
    Lane 2 : mouse brain lysate
    Lane 3 : Ramos cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HL60 cell lysate
    Lane 6 : Hela cell lysate


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab59988 at 1/50-1/100 dilution staining Chk1 by Immunohistochemistry on Formalin-fixed, Paraffin-embedded Human hepatocarcinoma tissue.
  • ab59988 staining phosphoChk1 (S280) in human colon adenocarcinoma cells (LoVo) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% and blocked with 1% BSA for 2 hours at 20°C. Samples were incubated with primary antibody (1/250 in PBS + 0.1% Triton) for 3 hours at 20°C. A 1.4 nm Gold (ultra-small) FITC-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

Anti-Chk1 (phospho S280) antibody (ab59988) 使用論文

ab59988 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HCT116)
Specification
HCT116
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Dec 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (human colon cancer cells)
Loading amount
25 µg
Specification
human colon cancer cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

投稿 Apr 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human mammary cells)
Loading amount
50000 cells
Specification
Human mammary cells
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. pierre laetitia

Verified customer

投稿 Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cancer mammary cell)
Specification
cancer mammary cell
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0,5%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human colon adenocarcinoma cells (LoVo))
Specification
Human colon adenocarcinoma cells (LoVo)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton 0.1%
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human colon adenocarcinoma cells (LoVo))
Loading amount
20 µg
Specification
Human colon adenocarcinoma cells (LoVo)
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7)
Loading amount
35 µg
Specification
MCF7
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cell line)
Loading amount
20 µg
Specification
Colon cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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