製品の概要

  • 製品名Anti-Chk1 (phospho S280) antibody
    Chk1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chk1 (phospho S280)
  • 特異性This antibody detects endogenous levels of Chk1 only when phosphorylated at serine 280.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 280 (V-T-SP-G-G).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
ELISA 1/20000.

ターゲット情報

Anti-Chk1 (phospho S280) antibody 画像

  • All lanes : Anti-Chk1 (phospho S280) antibody (ab47560) at 1/500 dilution

    Lane 1 : HT29 cells, untreated
    Lane 2 : HT29 cells treated with UV


    Predicted band size : 54 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

Anti-Chk1 (phospho S280) antibody (ab47560) 使用論文

ab47560 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"