製品の概要

  • 製品名Anti-Chk1 antibody
    Chk1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Chk1
  • 特異性This antibody detects endogenous levels of total Chk1 protein.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic non phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280.

  • ポジティブ・コントロール
    • Extracts from HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab47444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ELISA
  • 追加情報ELISA: 1/1000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 54 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • Information by UniProt
    • 参照データベース
    • 別名
      • C85740 antibody
      • Cell cycle checkpoint kinase antibody
      • Checkpoint , S. pombe, homolog of, 1 antibody
      • Checkpoint kinase 1 antibody
      • Checkpoint kinase 1 homolog (S. pombe) antibody
      • CHEK 1 antibody
      • Chek1 antibody
      • Chk 1 antibody
      • Chk1 antibody
      • CHK1 checkpoint homolog (S. pombe) antibody
      • CHK1_HUMAN antibody
      • EC 2.7.11.1 antibody
      • rad27 antibody
      • Serine/threonine protein kinase Chk1 antibody
      • Serine/threonine-protein kinase CHK1 antibody
      • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
      see all

    Anti-Chk1 antibody 画像

    • All lanes : Anti-Chk1 antibody (ab47444) at 1/500 dilution

      Lane 1 : HeLa cells lysate
      Lane 2 : HeLa Cells Lysate - for this panel, ab47444 was pre-incubated with the immunizing peptide (negative control)


      Predicted band size : 54 kDa
    • ab47444 (1/200) staining Chk1 in assynchronous HeLa cells (green). Cells were fixed in methanol (-20oC, 10min) and counterstained in DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

      See Abreview

    Anti-Chk1 antibody (ab47444) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human mammary cells)
    Loading amount 50000 cells
    Specification human mammary cells
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 22 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MCF 7 human breast adenocarcinoma cell line)
    Loading amount 35 µg
    Specification MCF 7 human breast adenocarcinoma cell line
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 14 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Nov 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"